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At-home breath collection kit | Measures hydrogen, methane, and hydrogen sulfide gas
The Trio-Smart® Breath Test is the most advanced diagnostic tool available for identifying Small Intestinal Bacterial Overgrowth (SIBO). It is the only test clinically validated to measure all three key fermentation gases: hydrogen (H₂), methane (CH₄), and hydrogen sulfide (H₂S), giving a complete picture of microbial activity in the small intestine.
Unlike traditional 2-gas breath tests, Trio-Smart provides insight into less commonly identified gas patterns that are often responsible for unresolved bloating, constipation, diarrhea, food sensitivity, and chronic inflammation.
What This Test Detects:
Hydrogen SIBO – associated with bloating, loose stools, fatigue
Methane SIBO (IMO) – associated with constipation, slow motility, and gas retention
Hydrogen Sulfide SIBO – associated with sulfur sensitivity, loose stools, mood issues, and neurologic symptoms
Who This Test Is For:
This test is ideal for individuals experiencing:
Bloating or visible abdominal distention
Constipation, diarrhea, or alternating bowel patterns
Food sensitivity (especially to fiber, garlic/onion, or FODMAPs)
Brain fog, mood swings, or fatigue after meals
Histamine intolerance, skin flares, or incomplete relief from gut protocols
Suspected or previously diagnosed SIBO or IBS
What’s Included:
Trio-Smart® at-home breath collection kit
Lactulose substrate and detailed instructions
Complete lab analysis of hydrogen, methane, and hydrogen sulfide gas levels
Return shipping to Gemelli Biotech
Comprehensive Results & Wellness Review with our team
– Functional interpretation of gas patterns
– Personalized treatment considerations based on your findings
Important Note: Please follow all pre-test dietary and preparation instructions carefully for accurate results. This test is for wellness and educational purposes and is not intended to diagnose or treat medical conditions.
HSA/FSA Eligible - Check with your plan administrator, as many Health Savings Accounts and Flexible Spending Accounts allow reimbursement for this testing service.

